产品详情介绍
本产品以RNA为模板，在同一反应管内，用TransScript® RT/RI Enzyme Mix合成第一链cDNA的同时去除RNA模板中残留的基因组DNA。反应结束后，只需在85℃加热5秒钟，即可同时失活TranScript® RT与gDNA Remover。
• 在同一反应体系中，同时完成反转录与基因组DNA的去除，降低RNA污染机率。
•产物用于qPCR：反转录15分钟；产物用于PCR：反转录30分钟。
•反应结束后，同时热失活RT/RI与gDNA Remover。与传统的用DNase I 预处理RNA 的方法相比，避免了处理后热失活DNase I对RNA 的损伤。
• 操作简单。
• 合成片段≤ 12 kb。
使用前请将各组分点甩离心
产品稳定性
使用不同批次产品分别以人总RNA为模板，进行RT-PCR检测，1.0%琼脂糖凝胶电泳分析反转录效果。
使用不同批次产品分别以人100 ng总RNA、人100 ng总RNA+200 ng gDNA、200 ng gDNA为模板，进行RT-PCR检测，1.0%琼脂糖凝胶电泳分析模板DNA去除效果；qRT-PCR检测18S RNA表达量。
与竞品的比较
使用TransGen、Company TA和Company TH产品，分别以人100 ng总RNA、人100 ng总RNA+200 ng gDNA、200 ng gDNA为模板，进行RT-PCR检测， 1.0%琼脂糖凝胶电泳分析模板DNA去除效果；qRT-PCR检测18S RNA表达量。
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