产品详情介绍
本产品含有反转录反应所需的全部试剂(TransScript® Uni RT, RNase Inhibitor, Anchored Oligo(dT)20 Primer, Random Primer(N9), dNTPs, Buffer),浓度为5×。反应时,只需加入gDNA Remover、模板RNA和水,在42℃-65℃条件下即可高效地合成第一链cDNA,同时去除RNA模板中残留的基因组DNA。另配有5×TransScript® Uni All-in-One No-RT Control SuperMix for qPCR,用于配制无反转录酶的对照,判断qPCR模板是否来自cDNA。该产品操作简便,降低操作过程中的污染机率。cDNA只适用于qPCR,不适用于常规PCR。
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